Lately I switched to μ-chain-specific secondary antibodies for IgM detection and got cleaner bands, but also a weird drop in signal on older samples. It took me back to a late night in the lab chasing ghost bands. Has anyone else run into unexpected trade-offs like that?
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From my side, the switch felt subtle at first, then kind of eye-opening. Using μ-chain-specific secondaries reduced cross-reactivity in my assays, but it also made sample prep feel less forgiving. I had to rethink how fresh my controls were. I even caught myself rereading basics about what IgM really is, including the lgm full form, just to reset my thinking. It didn’t magically fix anything, but it definitely changed how I read weaker signals now.